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Concours piwi nuage


AU - Nagao, Akihiro, aU - Sato, Kaoru AU - Nishida, Kazumichi.
Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208.
At the end of mitosis, Piwi leaves nuage to return to the nucleus.
Authors: J Cell Biol ;213(2 201-11.Nagao, Akihiro ; Sato, Kaoru ; Nishida, Kazumichi.We found that the Spn-E protein physically associates with the Tudor domain protein Qin and the piwi proteins Aubergine (Aub) and Argonaute3 (Ago3).Nagao A, Sato K, Nishida KM, Siomi H, Siomi.We suggest that this is in some way coupled with the execution of the piRNA amplification cycle.And Haruhiko Siomi and Siomi, Mikiko.AU - Siomi, Haruhiko AU - Siomi, Mikiko.gender-specific hierarchy in nuage localization of piwi-interacting RNA factors in Drosophila.CC-BY.0 International license.PY - 2011 Y1 - 2011 N2 - piwi-interacting RNAs (piRNAs) are germline-specific small code promo forfait les saisies non-coding RNAs that form piRNA-induced silencing complexes (piriscs) by associating with piwi proteins, a subclade of the Argonaute proteins predominantly expressed in the germline.April 2016 6 Reads, publication Analysis, top Keywords nuage granules 16 piwi proteins 12 qin piwi 8 amplification cycle 8 mutually dependent 8 nuage 6 spn-e 5 protein qin 4 domain protein 4 tudor domain 4 associates tudor 4 spn-e qin 4 proteins aubergine.Then, press proceed to send the authors a message.Copyright, the copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.Here, we show by immunostaining of both ovaries and testes from piRNA factor mutants that the molecular hierarchy of piRNA factors shows gender-specificity, especially for Krimper (Krimp a Tudor-domain-containing protein of unknown function(s Krimp is dispensable for piwi protein Aubergine (Aub) nuage localization in ovaries.One cofactor required for amplification is the helicase Spindle-E (Spn-E).Transposable elements (TEs) are silenced in germ cells by a mechanism in which piwi proteins generate and use piwi-interacting ribonucleic acid (piRNA) to repress expression of TE genes.
PiRNA biogenesis occurs by an amplification cycle in microscopic organelles called nuage granules, which are localized to the outer face of the nuclear envelope.


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